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Biolot human embryonic lung fibroblasts (helf)
Human Embryonic Lung Fibroblasts (Helf), supplied by Biolot, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+lung+fibroblasts+(helf/human+embryonic+lung+fibroblasts++helf+/pm37568583-111-0-13
Average 90 stars, based on 1 article reviews
human embryonic lung fibroblasts (helf) - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Imidazole-4-N-acetamide Derivatives as a Novel Scaffold for Selective Targeting of Cyclin Dependent Kinases.
Article Snippet: Human embryonic lung fibroblasts (HELF) and mesenchymal stem cells (MSC) were purchased from Biolot, Saint-Petersburg, Russia.

Article Title: Synthesis of a rare-earth doped hafnia hydrosol: Towards injectable luminescent nanocolloids.
Article Snippet: Accepted Manuscript Title: Synthesis of a rare-earth doped hafnia hydrosol: Towards injectable luminescent nanocolloids Author: Aleksandra D. Furasova Anna F. Fakhardo Valentin A. Milichko Elena Tervoort Markus Niederberger Vladimir V. Vinogradov PII: S0927-7765(17)30101-7 DOI: http://dx.doi.org/doi:10.1016/j.colsurfb.2017.02.029 Reference: COLSUB 8401 To appear in: Colloids and Surfaces B: Biointerfaces Received date: 6-9-2016 Revised date: 27-1-2017 Accepted date: 21-2-2017 Please cite this article as: A.D. Furasova, A.F.. Fakhardo, V.A.. Milichko, E. Tervoort, M. Niederberger, V.V.

Article Title: Optically Active Hybrid Materials Based on Natural Spider Silk.
Article Snippet: Spider silk is a natural material possessing unique properties such as biocompatibility, regenerative and antimicrobial activity, and biodegradability.. It is broadly considered an attractive matrix for tissue regeneration applications.. Optical monitoring and potential control over tissue regrowth is an attractive tool for monitoring this process.

Article Title: Imidazole-4-N-acetamide Derivatives as a Novel Scaffold for Selective Targeting of Cyclin Dependent Kinases
Article Snippet: Human embryonic lung fibroblasts (HELF) and mesenchymal stem cells (MSC) were purchased from Biolot, Saint-Petersburg, Russia.

Article Title: The controllable destabilization route for synthesis of low cytotoxic magnetic nanospheres with photonic response
Article Snippet: To evaluate the cytotoxicity of 80 nm MNS the human non-malignant embryonic lung fibroblasts (HELF) and tumor HeLa cell lines (Biolot, St.Petersburg, Russia) were maintained in Eagle’s medium (Biolot) supplemented with 10% fetal bovine serum (Gibco) and 50 μ g/mL gentamycin (Biolot) at 37 °C, 5% CO 2 .

Article Title: Thrombin@Fe 3 O 4 nanoparticles for use as a hemostatic agent in internal bleeding
Article Snippet: Human embryonic lung fibroblasts (HELF) and HeLa cells obtained from Biolot (Saint-Petersburg, Russia) were maintained in Eagle’s medium (EMEM, Biolot), supplemented with 10% fetal bovine serum (FBS, Gibco) and gentamicin 50 μ g/mL (Biolot) at 37 °C and 5% CO2.

Article Title: Synthesis of upconversion zirconia nanoparticles for bioimaging
Article Snippet: The amount of surviving HeLa and human embryonic lung fibroblasts (HELF) (Biolot) cells was determined indirectly by measuring the MTT dye reduction by NAD(P)H dependent cellular oxidoreductase enzyme [16].

Derivative Assay:

Article Title: Low dose ionizing irradiation suppresses cellular senescence in normal human fibroblasts.
Article Snippet: Purpose: Exposure to high dose ionizing radiation leads to premature cell senescence and suppression of cell proliferation.. In contrast, low dose and low dose-rate gamma-irradiation can lead to stimulation of cell proliferation.. We aimed to examine whether the low dose radiation-induced proliferation of normal human fibroblasts can lead to a progressive depletion of proliferation potential and to an early onset of senescence.



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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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https://www.bioz.com/product/human+embryonic+lung+fibroblasts+(helf/Primary+Lung+Fibroblast%3B+Fibrosis/pm36463525-34-14-22
Average 97 stars, based on 1 article reviews
human embryonic lung fibroblasts helf - by Bioz Stars, 2026-10
97/100 stars
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Embryonic lung fibroblasts stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates

Journal: Stem Cell Research & Therapy

Article Title: Influence of mesenchymal and biophysical components on distal lung organoid differentiation

doi: 10.1186/s13287-024-03890-2

Figure Lengend Snippet: Embryonic lung fibroblasts stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates

Article Snippet: Human embryonic lung fibroblasts (hELF, 17.5 weeks of gestation, DV Biologics) were cultured in DMEM (Gibco, #11965118) containing 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin.

Techniques: Co-Culture Assay, Irradiation, Flow Cytometry, MANN-WHITNEY